Differnce Between Online and Physical Shopping

Introduction Online shopping or online retailing is a form of electronic commerce whereby consumers directly buy goods or services from a seller over the Internet without an intermediary service. An online shop, eshop, e-store, Internet shop, webshop, webstore, online store, or virtual store evokes the physical analogy of buying products or services at a bricks-and-mortar retailer or shopping centre As we know that online shopping is the easy way shopping but also there are certain advantages as well as disadvantages.To overcome the technological challenges as well as global challenges most of the business organizations are running towards ecommerce or e-business. For the shopping of the two items I choose malla emporium and muncha. com to buy Saree and amazon. com and GS electronics to buy watch. While going to shopping I was unknown that what kind of saree to buy so I went in muncha. com for online shopping of saree . There I got different types of saree which can fulfill my requir ement. So I choose chiffon saree with blouse set. After then I moved to malla emporium for shopping of same that type of saree.There they show almost all kind of saree but I couldn’t find the saree like that which I got in muncha. It was so difficult to select saree and quality of it and while selecting there is a loss of time also but while doing by online it was easy and less time consuming. Another good that I would like to buy was watch. For watch I went to amazon. com which is a much known online business site. When I moved to amazon. com I was confused that which kind of watch I should buy. So I search watch at first then I saw titan watch which I like very much so I select that.To buy watch I went to GS electronics where there is showroom of watch. There I looked for the same watch but it was too difficult. I got titan watch but I couldn’t get the same one and the price rate was so different. 2. Shopping Comparison between Online and Physically In this world hum an being always prefer change. And by keeping in view this thing, there is another drastic change we see in shopping. Now a day we saw two types of shopping. The first is done by physically and the second is done by online. Attributes of shopping modes CharacteristicsAttributes Physical Shopping Online Shopping Information/Gathering ShoppingTravel cost- When I go to malla emporium for shopping there is a requirement of travel cost. No travel cost- While I do shopping through ebay, travel cost is not required. I can get a service in one click. Travel time- In the physically shopping we’ve to reach upto that store so there is loss of time. No travel time- As we do shopping by online then we can do shopping by one click in our home itself so time will be saved. More shopping fun- person have different view point.So while doing shopping physically we can enjoy. Less shopping fun- Online shopping is done by one click staying at a place so it’s not so fun. Less information c ertainity- That’s not sure that every shopkeeper tells the truth about the product so we cannot get full information about the product. More information certainity- We can get the real information that we want about the products in online shopping. Purchase/ TransactionHigher Purchase Price- When we do shopping by physically we’ve to charge high price because there is hidden cost like rental, inventory, labor cost.Lower purchase price- Online shopping help us to get a services in a reasonable price then physically shopping. Less distrust feelings caused by transactions. More distrust feelings caused by transactions. Transactions are mostly made by cash but in some place there are used of visa, debit cards. Transactions only occurred through master cards, credit cards, visa, debit card. Delivery timeNo delivery time- We can get the services by hand to hand so no delivery time is required.Delivery time- While doing shopping through online there is requirement of time for delivering of goods to us. Less inconvenience caused by delivery. More inconvenience caused by delivery. 3. Conclusion/ Recommendation Based on the project leading individuals to reallocate their time and money resources, this study examined the time and cost attributes of shopping modes, and explored the tradeoff between these two attributes, i. e. , the value of time, by assuming that consumers were faced with a shopping mode choice between physical store shopping and e-shopping.The final estimated value of time include two types: the value of travel time to shopping places, physical stores as far as this study concerned, and the value of waiting time for the delivery of purchased products. Of course consumers’ concern toward e-shopping is not only about time and cost. Some psychological aspects, such as information uncertainty and transaction security, have been playing an important role in dominating consumers’ e-shopping behavior, and have been even more widely d iscussed in the literature.However, ecommerce continues to advance, in speed and security in particular, it is generally believed that online information will be to a great extent improving both in quantity and quality in the near future. By that time, consumers’ negative perceptions towards e-shopping, such as information uncertainty and transaction security, may fade away. If this is going to be true, then consumers’ psychological concern over e-shopping may gradually be disappearing in the future. On the other hand, the economic concern over the travel problem; i. . , travel time and travel cost, about physical store shopping, and product delivery problem about e-shopping will ever exist. This makes the value of travel time is more costly then the value of delivery time, this study estimates worth noting. Moreover, this study also found that purchasing online to save travel time and travel cost, which is worth more for avoiding a shopping trip can be very inviting t o consumers, even though it is at the cost of waiting for a delivery of purchased products, which is worth an average monetary value.While delivering goods there arises problem. Delivery charge differs according to the goods. First, the value of product delivery time seems to highly depend on the types of products consumers shop and purchase. After all, waiting for a delivery of saree may take more time which I need for the especial party and also while delivering watch also takes time which I had to gift for the birthday f my brother.But also this delivery time is reduce by this online business sites according to the products. According to this project I come to know that online shopping is better for shopping rather than physically shopping because there is saving of time, money and we can get the more information about the products and services which we don’t get from the physically shopping. In the case of security there is strict rules and regulations which help us to ge t the product safely.

Chondrogenesis Of Adult Stem Cells Health And Social Care Essay

1.0 IntroductionDegenerative gristle diseases, such as degenerative arthritis ( OA ) , affect 1000000s of people worldwide and therefore have tremendous societal and economic effects. Osteoarthritis is characterized by progressive devastation of articular gristle and finally the full articulation, associated with variable grades of local redness, motional hurting, puffiness of the damaged tissue and thickener of the articulations. The internal fix capacity of articular gristle is hapless due to several alone features of the tissue, including slow turnover of gristle collagen, deficiency of vascularisation and the low figure of root cells that could lend to the fix. In mature articular gristle, chondrocytes have small intrinsic potency for fix. Repair may happen by an extrinsic mechanism, which depends on mesenchymal stromal cells ( MSCs ) in next connective tissue. Several intervention options have been used for gristle fix in gristle lesions, including soft tissue trades, and chondrocyte and osteochondral organ transplant. However these methods are limited by the hapless handiness of suited donor tissue and the hazard of infection and implant failure associated w ith entire joint replacings [ 14 ] . These restrictions are the driving force behind much research into cell-based methods for efficaciously handling diseased or damaged gristle [ 15 ] . Tissue technology has been defined as â€Å" an interdisciplinary field that applies the rules of technology and the life sciences toward the development of biological replacements that restore, maintain, or better tissue map † [ 14 ] ) . Tissue technology schemes by and large involve the undermentioned phases: ( 1 ) designation and isolation of a suited beginning of cells ; ( 2 ) industry of a device to either carry or encapsulate the cells ; ( 3 ) uniform seeding of cells onto or into the device and appropriate civilization ; and ( 4 ) in vivo nidation of the engineered concept ( Figure-1 [ 16 ] ) . Figure-1: Conventional representation of a tissue technology scheme: ( A ) isolation of an appropriate cell population ; ( B ) fiction of a scaffold ; ( C ) seeding of cells into scaffold and in vitro civilization of cell-scaffold concept ; and ( D ) nidation of tissue engineered device. The field of tissue technology has opened up new possibilities for fix and regeneration of gristle by uniting cells, biomaterial scaffolds, and bioactive signals [ 1, 2 ] . Mesenchymal cells ( MSCs ) offer a promising beginning due to its ability to proliferate extensively and distinguish into multiple cell line of descents in vitro and in vivo, including chondrocytes, bone-forming cells and adipocytes [ 6 ] . MSCs are present in a assortment of grownup tissues, such as bone marrow and adipose tissue. Adult root cells derived from adipose tissue ( adipose-derived root cells, ASCs ) [ 3,4 ] and bone marrow ( bone marrow-derived mesenchymal root cells, BMSCs ) [ 5,6 ] have shown important chondrogenic potency for such a tissue technology attack [ 7-10 ] . The BMSCs or human bone marrow stromal root cells can be cultured, expanded and so transplanted into the injured site or, after seeding on molded polymer scaffolds, placed back in the patient to bring forth appropriate tissue concepts. It is a instead clip overwhelming process for the patients and the research lab employees. Besides, these BMSCs have a low cell figure upon reaping so they need to be expanded and so transplanted to the injured site or seeded on/in a polymer scaffold. The procedure takes much clip as besides the clip it requires in the research lab, the patients need to be operated twice, first to roll up the BMSCs and-or stabilise the defect and so to put the scaffold or transfer the BMSCs. Adipose tissue might be a promising alternate beginning of root cells that could hold far-reaching effects on several Fieldss including gristle tissue technology. It is really good known that root cells derived from adipose tissue are capable to distinguish into adipocytes, chondrocytes, bone-forming cells and myoblasts, like Mesenchymal Stem Cells ( MSCs ) . ASCs have attracted involvement due to ease of isolation process and comparative copiousness handiness of cells as compared to BMSCs [ 11, 12 ] . Large figure of ASCs in adipose tissue makes them an ideal campaigner for so called â€Å" one measure surgical process † for the intervention of osteo chondral defects [ 13 ] . Chondrogenic distinction of these cells may be induced by specii ¬?c cytokines, growing factors [ 22 ] , biophysical stimulation such as hydrostatic force per unit area, hyperosmolarity, hypoxia and proviso of a suited three-dimensional ( 3-D ) environment. In this literature describe the focal point is on methods used for bring oning adipose derived root cells and bone marrow derived root cells into the chondrogenic line of descent and besides to know apart initiation methods between adipose derived root cells and bone marrow derived root cells. This leads to treatments on ( 1 ) rudimentss of gristle tissue technology and the usage of root cells ( 2 ) features and biological facets of BMSCs ( 3 ) features and biological facets of root cells derived from adipose tissue ( 4 ) difference between these two root cells, ( 5 ) Factors used for bring oning adipose root cells in to chondrogenic line of descent2.0 Cartilage2.1 Cartilage biological scienceCartilage is a an avascular, aneural and alymphatic connective tissue nowadays in many sites of the craniate organic structure, including the jointing articulations between castanetss, rib coop, ear, nose, bronchial tubings and intervertebral phonograph record. Cartilage is classified in three s ubtypes based on its morphology and the composing of the extracellular matrix ( ECM ) asA hyaloid gristle, elastic cartilageA andA fibro-cartilage. Out of the three subtypes hyaloid gristle is rich in collagen type II and proteoglycan and it is found in between articulations. Besides it is the lone gristle subtype that can prolong mechanical strain. Articular gristle, which is present at the jointing surfaces of castanetss within synovial articulations, is formed from hyaloid gristle. The location of articular gristle within the articulatio genus articulation is shown in Figure 2. Articular gristle forms a lasting bed with a thickness of 0.5 to 7.0mm at the surface cut downing clash between the castanetss and administering tonss across the full articulation surface [ 89 ] . The one disadvantage of gristle is that unlike other type of tissues, foods are non supplied by blood vass alternatively diffuse through the matrix and gristle is repaired at a slower rate than other connective t issues. This is due to the deficiency of the three-step lesion mending procedure nowadays in other tissues ; i.e. redness, proliferation and tissue remodeling. Chondrogenesis is the procedure by which gristle is formed from condensed mesenchymal connectiveA tissue, which differentiates intoA chondrocytesA and secretes the molecules that form the extracellular matrix. The procedure begins with the collection and condensation of loose mesenchyme. Early in foetal development, the greater portion of the skeleton is cartilaginous. ThisA temporaryA gristle is bit by bit replaced by bone ( Endochondral ossification ) , a procedure that ends at pubescence. In contrast, the gristle in the articulations remains unossified during the whole of life and is, hence, A permanent. Factors such as the bone morphogenetic proteins ( BMPs ) are known to play critical functions in the compression of mesenchymal cells and the defining of the condensation [ 17 ] . As discussed before, onceA damaged gristle has limited fix capablenesss sinceA chondrocytesA are bound inA blank, they can non migrate to damaged countries. Fig-2 Location of articular gristle within the articulatio genus articulation ( adapted from Drury and Shipley 1998 ) .2.2 Cartilage tissue technologyAs gristle healing is limited, there exists a turning demand for cell based schemes for gristle fix. The quickly rising field of tissue technology holds great promise for the coevals of functional tissue replacements, including gristle, by technology tissue constructsA in vitroA for subsequent implantationA in vivo. The basic rule is to use a biocompatible, structurally and automatically sound scaffold that is seeded with an appropriate cell beginning, and is loaded with bioactive molecules to advance cellular distinction and/or ripening. Although advancement has been made late in technology gristle of assorted forms and sizes for decorative intents [ 18 ] , the challenges of technology a weight-bearing tissue, such as articular gristle that consists of multiphasic cellular architecture, are important. Articular gristle provides its ain peculiar challenges for tissue technology. Though its construction appears simple and contains merely one cell type, it has a complex and extremely organized extracellular matrix ( ECM ) . The physical belongingss of articular gristle depend on the construction and organisation of the supermolecules in the ECM. The construction of collagen gives it impressive tensile belongingss, which is utile to bring forth a tissue that is non merely strong in tenseness but besides immune to compaction. This is achieved by make fulling the interfibrillar matrix with a really high content of proteoglycan, chiefly aggrecanA [ 19, 20 ] . The challenge for gristle tissue technology is to bring forth gristle tissue with suited construction and propertiesA ex vivo, which can be implanted into articulations to supply a natural fix that with clip, will go incorporate with the patient ‘s tissues. There have been a figure of successful attacks to weave applied scientist gristle, including the usage of natural and man-made biomaterial scaffolds, allogeneic and autologous beginnings of mature chondrocytes and chondroprogenitor cells, biophysical cues such as hydrostatic force per unit area, hyperosmolarity, hypoxia, chondroinductive growing factors, such as the transforming growing factor-I?s ( TGF-I?s ) , and combinations thereof. Some of the current progresss in cartilage tissue technology are highlighted here.3.0 Characterization and biological facets of Adipose root cells and bone marrow root cells3.1 Bone marrow-derived root cells ( BMSCs )Bone marrow contains three types of root cells, haematopoietic root cells, m esenchymal root cells and endothelial root cells. Hematopoietic root cells give rise to the three categories of blood cells that are found in the circulation white blood cellsA ( leucocytes ) , A ruddy blood cells ( red blood cells ) , andA plateletsA ( platelets ) . Mesenchymal root cellsA are found arrayed around the cardinal fistula in the bone marrow. They have the capableness to distinguish intoA bone-forming cells, chondrocytes, A myocytes, and many other types of cells. Stem cells obtained from bone marrow are found in the stroma of the marrow. These cells are typically obtained from bone marrow aspirates from marrow graft givers. When cultured in vitro, bone marrow root cells exhibit a fibroblast-like morphology. Marrow stromal cells have been studied and certain cell surface markers have been identified which are utile in cell choice and finding of readying of marrow root cell populations [ 21 ] . In add-on to their ability to distinguish into multiple cell line of descents, the usage of bone marrow root cells offer a beginning of cells that is isolated and expanded in vitro. Bone marrow-derived root cells were isolated and described for the i ¬?rst clip by Friedenstein and Owen at the University of Oxford, UK, in the 1960s, followed by intensive work on the survey of the belongingss of these cells [ 23-29 ] . They took bone marrow and incubated for 4 hours in fictile civilization dishes and removed the non-adherent cells. A heterogonous population of cells was retrieved, with some adherent cells exhibiting a spindle form ; besides, they multiplied quickly in vitro, organizing distinguishable settlements called colony-forming unit i ¬?broblasts ( CFU-Fs ) . Finally, these cells were successfully isolated from several mammals, including worlds [ 30,31 ] . In add-on, the CFU-Fs were found to hold multi-potent, therefore connoting that they have stem cell belongingss [ 29,32 ] . These cells differentiated into multiple mesenchymal cell line of descents in vitro, including bone [ 33 ] , ligament [ 34 ] , adipose [ 33 ] , gristle [ 35,36 ] and musculus [ 3 7 ] . Similarly, following the organ transplant of CFU-Fs in vivo, they formed little sedimentations of bone, gristle or fat [ 37-39 ] . This was further supported the multi-potent potency of CFU-Fs. Similar cells have been isolated from different mesenchymal tissues, including synovial [ 40 ] , sinews [ 41 ] , skeletal musculuss [ 42 ] and adipose tissue [ 43,44 ] . BMSCs are normally isolated from the mononucleate bed of bone marrow after separation by denseness gradient centrifugation. These mononucleate cells are cultured in media incorporating 10-15 % foetal calf or autologous serum [ 45 ] . The BMSCs adhere to the tissue civilization plastic, go forthing little adherent i ¬?broblast-like cells. Thereafter, the cells divide and proliferate quickly. For in vitro chondrogenic distinction MSCs are needed to be kept in high denseness 3-D environment. This can be attained by aggregating the cells in mircomass pellets or as suspension in alginate. Different man-made or biological scaffolds like agarose, collagen suspensions, fibrin gels and biopolymers can be used [ 46-48 ] . Alginate bead civilization is an first-class tool for chondrogenic distinction surveies in vitro. Alginate is a additive polyose which is soluble in aqueous solutions and cells can be homogenously suspended in it. It is cross-linked in presence of Ca or other bivalent ions to organize a polymerized hydrogel. Importantly, it can be easy resolubilized by a chelating agent ethylenediaminetetraacetate ( EDTA ) to divide cells from the ECM [ 49 ] . Chondrogenic distinction is a complicated procedure necessitating good defined conditions, hence, external foetal calf serum ( FCS ) usually used for proliferation of MSCs has to be substituted by defined medium addendum, like widely used ITSa„? . ITSa„? contains insulin, beta globulin, selenious acid and linoleic acid suspended in solution of bovine serum albumen. Insulin as a endocrine addendum is necessary for endurance of cells as it is involved in fatty acid and animal starch synthesis. Transferrin is an iron-binding protein for endocrines and foods, hence, it is critical for in vitro cell growing and selenious acid is a cofactor for glutathione peroxidase necessary for cell membrane unity. Linoleic acid is an built-in constituent of chondrogenic medium, like many unsaturated fatty acids it is an built-in membrane constituent and of import for cell growing. It is non a specific chondrogenic distinction factor but in combination with Tgf-? has profound stimulatory consequence on chondrogenesis. Tgf-? entirely and in combination with Decadron enhance chondrogenesis [ 50,51 ] . The chondrogenic medium has to be enriched with proline because this amino acid is found in really high concen trations in extracellular matrices. Pro-x-gly-pro sequence motive appears really often in collagens where ten is normally a impersonal amino acid. Ascorbic acid is required as an negatron giver, therefore it contributes collagen synthesis by moving as a carbon monoxide factor for lysine proline hydroxylation necessity for the formation of typical-triple coiling collagen constructions [ 52 ] . As all the needed conditions are met MSCs start to lose their fibroblast like features and get down showing chondrocyte particular ECM which can be monitored by look of chondrogenic markers. Several other methods are used to assist distinguish MSCs into osteocytes, adipocytes, myocytes or tenocytes in vitro. Bone marrow aspirates are still being used though there are major restraints. The chief restraints in utilizing BMSCs are: heterogenous population of the cells, painful process to roll up the bone marrow and the population of MSCs nowadays in bone marrow is really low ( 0.001-0.01 % or about 1 MSC per 105 disciple stromal cells ) [ 53 ] . Besides MSCs needs to be expanded in vitro for a longer period of clip before adequate cells are present for seeding the scaffold or organ transplant [ 53 ] . These restraints favours the statement for placing new beginnings as feasible options to cram marrow, but farther surveies in clinically relevant animate being theoretical accounts are needed to better qualify the comparative – in our case- chondrogenic potency. One of these options might lie in the usage of root cells derived from adipose tissue.3.2 Adipose tissue-derived root cellsIn order to utilize adipose tissue for the usage of tissue technology, the composing of this tissue needs to be analyzed foremost. Adipose tissue is specialized connective tissue that maps as the major storage site for fat in the signifier of triglycerides. In grownup mammals, the major majority of adipose tissue is a loose association of lipid-filled cells, which are held in a model of collagen fibres. Adipose tissue, better known as fat is composed of blood cells, endothelial cells, pericytes, fibroblasts, adipose precursor cells, and adipocytes [ 54, 55 ] . Fat is divided into two types ( I ) br own adipose tissue ( BAT ) and white adipose tissue ( WAT ) . The morphology and map of brown fat cells are distinguishable from white adipocytes. The map of BAT is to interpret energy into heat production [ 56 ] . Phenotypically, brown fat cells are rich in chondriosome and accumulate lipoids in multiple little droplets [ 57 ] . The tissue what is normally recognized as â€Å" the fat † , which is besides the larger proportion of the organic structure fat, consists of WAT. Mature adipocytes of WAT are cells with one big lipid droplet and really small cytol with the karyon located at fringe of the cell [ 54 ] . This morphology is described as the signet pealing signifier and the cell may be termed a univacuolar adipocyte. White adipose tissue serves three maps: heat insularity, mechanical shock absorber, and most significantly, a beginning of energy [ 55 ] ( Fig 1 ) Figure 1a†Ã¢â‚¬Å¡ White fat cell and brown fat cell. Note the individual big lipid vacuole in the white fat cell and the legion smaller lipid vacuoles in the brown fat cell. LV: lipid vacuole ; M: chondriosome ; N: karyon. Artwork courtesy of Dr. John Horwitz, U.C. Davis. The white fat cells range in size from 25 to 200 micrometers. Mitochondrions are found preponderantly in the thicker part of the cytoplasmatic rim near the karyon. The big lipid droplet does non look to incorporate any intracellular cell organs. A brown fat cell may make a diameter of 60 micrometers and the lipid droplet within the cell may make 25 micrometers in diameter. The brown colour of this tissue is derived from the cells ‘ rich vascularization and dumbly jammed chondriosomes. These chondriosomes vary in size and may be round, egg-shaped, or filiform in form [ 56,57 ] .3.3 Biological facets of ASCs in vivo and in vitroThe exact beginning of the adipocyte, the chief cells of adipose tissue, is still non to the full understood. The earliest phase in adipocyte distinction is pluripotent root cells which give rise to mesenchymal precursor cells [ 58 ] . These pluripotent root cells are the adipose-derived grownup root ( ASCs ) cells. These ASCs are capable to distinguish in to the chondrogenic, osteogenic, and adipogenic line of descents, neural cells and clamber [ 58 ] . ASCs, besides called processed lipoaspirate cells ( PLA cells ) , are considered a separate cell population within the adipose tissue [ 4 ] . The capacity of ASCs to distinguish into several tissues suggests the presence of a root cell population within adipose tissue, it can non be ruled out though the presence of pluripotent cells from other beginnings, like marrow-derived MSCs from peripheral blood, or dedifferentiation of mature adipocytes will ensue in the coevals of pluripotent cells. However, the presence of MSCs in peripheral blood is non likely. The sum of MSCs in bone marrow is low, i1 MSC per 105 stromal cells [ 6,53 ] and the sum of MSCs in peripheral blood is even lower. One major advantage of ASCs is that it can be isolated from many fat terminals within the organic structure by minimally invasive suction lipectomy and by subsequent enzymatic digestion of adipose tissue [ 4 ] . Besides deficit of autologous donor tissue is, in the instance of adipose tissue, really improbable in most persons. ASCs are located in the stromal-vascular fraction ( SVF ) of the host adipose tissue. Adipose tissues are normally harvested by resection or suction lipectomy and later digested with collagenase type I. Once the stromal-vascular multitudes are isolated, they are cultured in lineage-specific civilization media. For chondrocyte distinction the cells are cultured in DMEM supplemented with FBS, insulin, transforming growing factor ( TGF ) , ascorbate and Decadron at suited concentrations. ASCs are able to defy freeze/thaw direction without any apparent impact on the growing characteristic. However, when adipose tissue is harvested from the organic structure the surgical processs used have some impact on the subsequent growing profile of ASC. Ultrasound-assisted suction lipectomy consequences in a lower frequence of proliferating ASC, every bit good as a longer population duplicating clip of ASC, compared with pure resection and puffy resection. Phenotypic markers are non affected by ultr asound intervention [ 59 ] . Guilak et Al. showed that ASCs ringers derived from lipoaspirates can distinguish along two or more of the adipogenic, osteogenic, chondrogenic and neuron-like cells [ 60 ] . These findings coupled with the fact that ASCs can undergo several population doublings while retaining their distinction capablenesss, supports the hypothesis that ASCs are a type of multi-potent grownup root cells [ 60, 61 ] . In vitro, distinction of ASCs cells can be induced by the supplementation of endocrines and substrates to the growing medium. Some illustrations are: Glucocorticoids ( corticosterone, hydrocortisone, dexamethason, and cortisol ) , phosphodiesterase inhibitors ( IBMX, forskolin ) , peroxisome proliferator-activated receptor ( PPAR I? ) ligands ( adipogenic distinction ) , indomethacin, fibrates ( Atromid-S, bezafibrate, and fenofibrate ) , insulin, and liothyronine. Besides growing factors have been found to modulate distinction, like basic FGF, EGF, PDGF, TGF I? , and TNF [ 44 ] . An illustration of lineage-specific distinction induced by media supplementation is demonstrated by Zuk et Al. [ 44 ] . The experimental and clinical grounds shows that ASCs are an equal alternate beginning for regenerative medical specialty peculiarly because it can be maintained in vitro for drawn-out periods of clip with stable population doublings and low aging degrees [ 4 ] . Although the bone marrow is a dependable beginning of root cells, its crop is an invasive process and the figure of stray cells can be low and age dependant. Pittenger et Al. [ 6 ] showed that merely 0.01-0.001 % of mononucleate cells isolated from the bone marrow lead to colony-forming units. On the other manus, adipose tissue can give big sums of root cells and can be obtained in copiousness. As such, ASCs open legion and promising positions for regenerative therapies.4.0 Factors impacting chondrogenesis4.1 Consequence of Growth factors on chondrogenesisThe usage of cytokines and growing factors is really of import for the development of a defined civilization environment for directing the chondrogenic distinction of root cells. Indeed, legion cytokines and growing factors have been implicated in chondrogenesis. As the procedure of chondrogenesis is so closely related with osteogenesis, many of the cytokines and growing factors that promote chondrogenic distinction are besides some what involved in osteogenic distinction [ 62, 63 ] . Hence, the challenge is to happen an optimized elusive combination of these assorted cytokines and growing factors that would assist in distinction specifically toward the chondrogenic line of descent. Among the most powerful inducers of chondrogenic distinction are members of the transforming growing factor beta ( TGF-I? ) household. Besides the assorted iso-forms of TGF-I? [ 64,66-67 ] and bone morphogenetic protein ( BMP ) [ 65,68 ] , other members of the TGF-I? ace household include activin [ 69 ] , osteogenic protein-1 [ 70 ] , and growing distinction factor 5 ( GDF-5 ) [ 71 ] are used. The effects of cytokines of the TGF-I? ace household on chondrogenic distinction are transduced through two major intracellular signaling tracts, SMAD household of signaling molecules [ 72 ] and involves mitogen-activated protein kinase ( MAPK ) signaling [ 73 ] . Interestingly, both signaling Cascadess are activated by the same TGF-I? receptor composite. Barry et al demonstrated that TGF-I? 2 and TGF-I? 3 iso-forms have stronger chondrogenic potency than TGF-I?1 [ 78 ] . Kim et Al showed that combinations of greater doses of presently known growing factors, TGF-I? 2 and IGF-I, lead to a chondr ogenesis from ASCs comparable to that observed with BMSCs [ 79 ] . Another household of cytokines that plays an of import function in chondrogenesis is comprised of assorted iso-forms of fibroblast growing factor ( FGF ) . The effects of FGF on chondrogenic distinction are transduced chiefly through MAPK signaling [ 74 ] . This leads to increased look of the written text factor Sox9, which is the maestro regulator of chondrogenesis. Besides the TGF-I? and FGF household of cytokines, insulin like growing factor-1 ( IGF-1 ) [ 75-77 ] has besides been shown to hold effects on chondrogenic distinction.4.2 Effect of biomaterial scaffolds ( biologic, biochemical and biomechanical belongingss ) on ChondrogenesisA broad scope of scaffolds have been used in gristle tissue technology surveies. Cartilage regeneration utilizing scaffolds involves seeding extremely porous biodegradable scaffolds with donor cells and/or growing factors. After seeding the scaffolds with the giver cells and/or grow ing factors, these scaffolds are implanted. Cells attached to the scaffold, so retroflex, distinguish into chondrogenic cells and form into normal healthy bone as the scaffold degrades. These scaffolds may be categorized with regard to the types of stuff used ( natural or man-made, degradable or non-degradable ) , the geometry of the scaffold ( gels, hempen meshes or porous sponges ) and their construction ( entire porousness, pore size, connectivity and distribution ; [ 103 ] . It is important that a tissue technology scaffold is fabricated from a stuff that is biocompatible, allows fond regard of cells, ECM secernment and tissue formation without the initiation of an inflammatory or toxic response [ 104 ] . In order for cells to be able to infiltrate the construction uniformly, it should incorporate a big figure of interrelated pores [ 105 ] . The size of the pores is of import to the infiltration and fond regard of the cells, for chondrocytes an optimal pore size of between 100 a nd 200I?m has been suggested [ 104 ] . The scaffold must besides be permeable, to let diffusion of foods into the matrix and the remotion of metabolic and degradation byproducts from it. Finally, it is of import that the scaffold has mechanical belongingss that allow it to defy nidation and the tonss experienced in vivo Many natural stuffs have been used because of their similarity with gristle ECM constituents, for illustration hyaluronan and collagen [ 106 ] . Other natural stuffs used in gristle tissue technology surveies include agarose, alginate and chitosan. Natural polymers are advantageous in tissue technology applications as they can undergo cell-specific interactions. The usage of natural stuffs, nevertheless, is limited by the big fluctuation between batches, the deficiency of big supplies for commercial usage and as they are frequently derived from non-human tissue they carry the hazard of reassigning pathogens [ 107 ] . Man-made polymers are frequently used in penchant to natural stuffs as it is possible to mass-produce polymers with custom-designed belongingss. Poly ( lactic acid ) ( PLA ) , poly ( glycolic acid ) ( PGA ) and co-polymers of PLA and PGA ( PLGA ) are normally used in tissue technology surveies as they have Food and Drug Administration ( FDA ) blessing for usage within the human organic structure. Ideally a scaffold that is to be implanted into the human organic structure should be biodegradable ) and the debasement merchandises should be non-toxic [ 104 ] .4.3 Effect of hypoxia on chondrogenesisOxygen lack within gristle tissues induces a hypoxic province which affects chondrocyte map. Cells exposed to hypoxia respond in many ways by impacting cistron look, distinction, and programmed cell death [ 91 ] . Transcription factor HIF-1I ± is considered as a survival factor for bring oning in hypoxic environments which inhibits proliferation but increases ECM production in mesenchymal ro ot cells ( MSCs ) and chondrocytes [ 92, 93 ] . Conditional omission of the cistron encoding HIF-1I ± in chondrocytes consequences in programmed cell death in hypoxic countries [ 92 ] and besides well reduces the chondrogenic possible [ 94 ] , proposing that hypoxia-induced up-regulation HIF-1I ± supports chondrogenic committedness. HIF-2I ± has besides been shown to command hypoxia enhanced chondrogenesis through both SOX9 dependant and independent signaling tracts [ 95 ] . Thus, hypoxia enhances the chondrogenic potency of mesenchymal cell populations during the development phase and under diseased conditions.4.4 Effect of hydrostatic force per unit area on chondrogenesisHydrostatic force per unit area has been proved to hold positive consequence on gristle in a figure of surveies [ 80 ] as exposure to increased hydrostatic force per unit area coincide with increased gristle thickness in vivo [ 81,82 ] . Cartilage is a extremely hydrous tissue, represent 70 % to 80 % H2O per moisture weight. Cartilage cells located in articular articulations experience hydrostatic force per unit area during periods of lading. The synovial fluid within the joint capsule transmits force per unit area to the H2O trapped within the gristle matrix, bring forthing a unvarying burden on chondrocytes in the tissue. The attractive force of negatively charged proteoglycan molecules within gristle causes its high H2O content, making a matrix that is composed chiefly of interstitial fluid. The swelling force per unit area of the proteoglycans is balanced by the tenseness of the collagen web, leting the tissue to swell significantly but still be a comparatively stiff stuff. High H2O content in the tissue plays a major function in the ability of the tissue to defy big compressive forces. Cartilage is incompressible under physiological degrees of hydrostatic force per unit area ( 7-10 MPa ) , ensuing in minimum tissue distortion [ 83-85 ] . Loading in this mode is comparatively safe for the structural unity of the tissue because it is a pure hydrostatic force per unit area that does non stretch or shear the tissue matrix. When gristle is loaded, the force is transmitted throughout the tissue, the fluid stage ab initio supports the applied burden, because H2O is trapped within the solid matrix of the tissue because of its low permeableness. The alteration in force per unit area from within the matrix finally forces fluid out of the tissue and into the synovial pit. The opposition that the fluid experiences as it leaves the matrix helps disperse energy and allows for a gradual softening of the tissue. This procedure efficaciously cushions the daze of a sudden burden without damaging the cells or extracellular matrix. The force per unit area that is produced by the tight fluid Acts of the Apostless uniformly on the chondrocytes within the matrix. This interstitial force per unit area, which for diarthrodial articulations ranges between 7 and 10 MPa during normal a ctivities [ 83,84 ] , is applied sporadically to the cells when walking, running, or switching weight while standing. Research workers emulated the mechanical stimulation in the research lab to engineer a higher quality gristle tissue [ 85-87 ] . The continuance and magnitude of hydrostatic lading vary widely as demonstrated by different surveies. Smith and associates exposed high-density chondrocyte monolayers to 10 MPa at 1Hz for 4h a twenty-four hours and obtained an addition of 65 % in GAG synthesis [ 88 ] . In another experiment utilizing the same lading regimen on cell monolayers, Smith and associates obtained a 9-fold addition in type II collagen messenger RNA and a 20-fold addition in aggrecan messenger RNA [ 89 ] . In a 3-dimensional experiment by Carver and Heath, concentrations of sulfated proteoglycans in civilized scaffolds were found to be twice every bit high as in controls after utilizing a 3.5-MPa, intermittent force ( 5/15 s on/off for 20 min every 4 H ) [ 90 ] . T hese consequences show that hydrostatic force per unit area is of import to chondrogenesis, particularly when cells are grown in a 3-dimensional environment.4.5 Effect of hyperosmolarity on chondrogenesisDuring mechanical burden, flow of H2O out of joint gristle will take to increase in osmotic force per unit area within the tissue. This indicates that osmotic force per unit area may positively act upon chondrogenesis. Osmotic force per unit area within the tissues additions due to the keeping of the extremely anionic proteoglycan and the necessary cationic counter ions and the osmotic force per unit area around the chondrocyte is changed straight by the burden on the tissue. Assorted surveies conducted to look into the effects of osmolarity on chondrocyte ECM synthesis shown that chondrocyte proteoglycan synthesis can be decreased through the application of hyper- or hypo-osmotic conditions [ 98,100,101 ] . Besides a survey conducted late explained that application of dynamic hypo- osmotic emphasiss resulted in increased look of gristle ECM cistrons [ 96 ] . The survey besides showed an addition in glycosaminoglycan synthesis by articular chondrocytes under hyperosmotic conditions [ 97 ] over 24h civilizations. It besides showed the demand for p38 mitogen-activated protein kinase ( MAPK ) signaling, a transduction pathway known to be regulated by osmolarity in many beings [ 99 ] . Besides Wouter et Al. in his recent survey demonstrated that the application of hyperosmolarity and hypoxia in combination, is able to bring on chondrogenesis in ASCs to the same degree as initiation with transforming growing factor I?1 [ 102 ] .5.0 Conclusion and future waiesThe usage of MSCs in gristle tissue technology applications is a powerful ( new ) tool in the development of clinical schemes for fix of site-specific gristle defects and will assist to handle assorted gristle upsets. Currently bone marrow aspirates are still considered to be the most accessible and enriched beginning of MSCs [ 6 ] . However, the sum of cells at initial crop are limited in bone marrow aspirations, which makes bone marrow non an optimum beginning of MSC [ 4,16 ] . Furthermore, the process of aggregation of bone marrow aspirates is a painful process and therefore other beginnings are preferred. As such adipose tissues have been identified as a beginning from which adequate chondrogenic primogenito r cells can be obtained at initial crop for research lab and clinical usage. It besides provides us an advantage of insulating adequate cells in a short sum of clip during tissue civilization enlargement. Adipose Stem Cells ( ASCs ) have the similar distinction potency, morphology, phenotype and cistron look as MSCs, as demonstrated by several surveies and are considered as a better beginning of multi-lineage mesoblastic root cells for tissue technology in regard to their copiousness and handiness [ 6,43 ] . In decision, we know that there are multiple root cell reservoirs available for gristle TE research and clinical applications ; we should see utilizing the reservoir that is ( I ) available in a big volume with limited morbidity of the environing tissue upon crop ; and ( two ) accessible without or with the lowest sum of hurting. Furthermore the reservoir should ( iii ) clasp cells which are capable of distinction into chondrocytes, produce gristle seeded on biomaterial like scaffolds and ( four ) can be detected by simple research lab methods. In my sentiment adipose tissue provides us menus better in comparing in all the above characteristics. However, to go on to take advantage of ASCs for gristle tissue technology and its applications we require a complete apprehension of how the care and distinction of ASCs are regulated both in vivo and in vitro. Though it is considered to hold greater possible and can be developed as a suited option to cram marrow, farther surveies in clinically relevant animate being theoretical accounts are needed for better proof. Besides regulative issues and safety facets should be addressed exhaustively in presymptomatic and clinical scenes. Standardization of protocols and sooner automated devices for isolation of ASCs need to be pursued with a proper set of surface markers to verify the presence of the right ASCs cells within the primary cell isolate. Future developments in biomaterial for scaffolds and bioreactor design for cell civilization will take without uncertainty to new vehicles for the bringing of ASCs to cartilage defect sites. Besides the development of optimum conditions such as h ypoxia and appropriate biophysical cues, hydrostatic force per unit area and hyperosmolarity for in vitro cultivation can further increase the chondrogenic committedness and the ability of these cells to intercede the coevals of de novo gristle tissue.

Benefits of Allowing Students to Bring Pets With Them to College

Benefits of Allowing Students to Bring Pets With Them to College To most of us, pets are family and we treat them as such. We give them the love and affection they show us daily. They know when we are sad, happy, or even stressed, and always know how to make our day better. If you’ve grown up with a family pet, it might be hard to leave them at home when you go off to college. Becoming a college student in general is very stressful and eye-opening, especially if you go out of state. Losing one of your best friends can be a tough transition to go through when going through that milestone. Many college students go through stages of depression and anxiety and have almost no way to cope with it. Allowing students to bring pets with them to college is beneficial to them by helping them cope with stress, anxiety, depression, and loneliness. Scientists have concluded many studies proving that animals improve your overall health and well-being. â€Å"A study of women in China found that those who were dogs owners had fewer doctor visits, took fewer days off sick from work, and exercised more often than non-dog owners† (Casciotti, Zuckerman). These results were found because pets, to a lot of owners, are like family and are treated as such. Pets always know when we are sad, happy, or scared. For example, some dogs love to greet their owners when they get home after a day of work, or cats love to purr and rub against you when they want your affection. Studies have shown that no matter your age or disability, pets can you make you feel like you have â€Å"a sense of responsibility and purpose that contributes to their overall well-being† (Csciotti, Zuckerman). Although pets can be very loving and caring, they can also be very messy and hard to keep up with. Depending on the animal, training them can be a daunting task. Cleaning up after them and making sure they arent getting into things they arent supposed to is a struggle for all pet owners. This is why many parents get their kids pets when they are young. Owning a pet teaches the individual about responsibility and what it is like to own something and take great care of it. For example, learning how to teach dogs tricks, how to behave properly, not to beg, and potty train them are just a few ways they can teach you responsibility (Kaye). As child, this can develop into life long skills and help you mature quicker. As a college student, this could help you with time manage ment and learning how to do things independently. College teaches you how to become independent and transition from childhood to adulthood. For some, this is never easy to do. There are always some kids who come to college and arent familiar with how to be independent and not have to rely on your parents all of the time. Many soon dropout due to the overwhelming stress, anxiety, and not being able to handle doing everything on their own. Students come to college and get very homesick as well. This may be the result of missing their family and pets. For most, the hardest thing to do when you leave for college is to leave your childhood pet at home. Transitioning into to adulthood can be a very stressful time in everybodys life. You have to rely on your own income to stabilize yourself and be independent on where you live and find a stable job. Studies have shown that owing a dog through early adulthood can help reduce stress and anxiety. Pet owners in the early 20’s also have a higher success rate in finding a stable job with a living income (Robinson, Segal). Pets can also serve as a companion when lonely. Some can even act as an audience when reciting a big presentation or stress reliever when finals are creeping up. Many students have tried to bring â€Å"Therapy Dogs† to college. Their reasoning is that their dogs help them reduce the stress and anxiety that many young students face daily. Some can even detect panic attacks before they happen and help calm their owner down (Martin). The number of people being diagnosed with depression and ADHD have risen dramatically over the years. The demand for Therapy Dogs have also increased in response. Depression is a growing problem in the teen/early adulthood community. Statistics have shown that students facing stress and loneliness in college are more likely to commit suicide and take their own life. One study showed how owning a pet can reduce loneliness and depression. Theres a reason dogs are â€Å"Mans Beet Friend† and thats because they act as a companion and show you love and affection. Their bright attitudes and soft, furry coat are proven to relieve any anxiety or depression you might have (Casciotti, Zuckerman). College can be a very hard transition for most teens. Most come into college with a preexisting depression disorder. Starting college can be difficult since you are away from home, on your own, and self-dependent. It has been proven that having a pet can help ones mood. From being sad, lonely, and depressed, the subject immediately became happy and excited when brought a dog (Nagengast). The same was done with a cat and the results didnt vary. A study given to California University states that 86% of their students would want to bring their pet to college or would like to have a pet for college (Martin). Some colleges have even agreed to do testing and studies by allowing some students to have a pet for a certain amount of time and compare their work ethic and behavior over those weeks compared to when they did not own a pet. The results showed how students with dogs were happier and more engaged in the classroom. They did better on tests and performed academically and athletically b etter. Their grades went up and became more engaged in the college community. Those who didnt have a pet were more likely to go out and party and put off studying. They would also fall asleep in class, do poorly on school work, and become more secluded (Martin). College teaches you how to become independent and transition from childhood to adulthood. For some, this is never easy to do. There are always some kids who come to college and arent familiar with how to be independent and not have to rely on your parents all of the time. Many soon dropout due to the overwhelming stress, anxiety, and not being able to handle doing everything on their own. Students come to college and get very homesick as well. This may be the result of missing their family and pets. For most, the hardest thing to do when you leave for college is to leave your childhood pet at home. Transitioning into to adulthood can be a very stressful time in everybodys life. You have to rely on your own income to stabilize yourself and be independent on where you live and find a stable job. Studies have shown that owing a dog through early adulthood can help reduce stress and anxiety. Pet owners in the early 20’s also have a higher success rate in finding a stable job with a living income (Robinson, Segal). Pets can also serve as a companion when lonely. Some can even act as an audience when reciting a big presentation or stress reliever when finals are creeping up. Many students have tried to bring â€Å"Therapy Dogs† to college. Their reasoning is that their dogs help them reduce the stress and anxiety that many young students face daily. Some can even detect panic attacks before they happen and help calm their owner down (Martin). The number of people being diagnosed with depression and ADHD have risen dramatically over the years. The demand for Therapy Dogs have also increased in response. Depression is a growing problem in the teen/early adulthood community. Statistics have shown that students facing stress and loneliness in college are more likely to commit suicide and take their own life. One study showed how owning a pet can reduce loneliness and depression. Theres a reason dogs are â€Å"Mans Beet Friend† and thats because they act as a companion and show you love and affection. Their bright attitudes and soft, furry coat are proven to relieve any anxiety or depression you might have (Casciotti, Zuckerman). College can be a very hard transition for most teens. Most come into college with a preexisting depression disorder. Starting college can be difficult since you are away from home, on your own, and self-dependent. It has been proven that having a pet can help ones mood. From being sad, lonely, and depressed, the subject immediately became happy and excited when brought a dog (Nagengast). The same was done with a cat and the results didnt vary. A study given to California University states that 86% of their students would want to bring their pet to college or would like to have a pet for college (Martin). When going to college it can be very beneficial to have a pet to bring with you. They help in many ways. They teach you responsibility, improve your mood, and help fight against depression, stress, and anxiety. It is clinically proven that owning a pet can dramatically improve your overall well-being. This can be crucial to a student during a tough time in their life. Having a pet can also decrease student suicides and even student dropout rate. Bringing pets to college will be very beneficial to the students and should be allowed in more colleges and universities.

Strategic Management - Southwest airlines Term Paper

Strategic Management - Southwest airlines - Term Paper Example The company’s success rests on the key pillars of value creation for its customers as well as employees. Of the entire strategic management, Southwest Airline’s key role in strategic human resource management has been an integral part. The company uses the words â€Å"LUV† and â€Å"FUN† to describe its relationship with employees as well as the deeper culture of the organization. Southwest believes in endowing employees with high respect as well as providing them with an environment that best supports their willingness to work. The company’s low turnover rates and high levels of productivity are indicators of its success. Another significant aspect of the company’s strong strategic position is its management of organization’s capabilities and resources. In this process, part of the value that is created for employees is translated into value addition for the shareholders and customers. Southwest’s source of competitive advantage lies in its delivery of value for money which is a combination of low operational costs and superior levels of customer satisfaction and service. The company, hence, is a model of generation of value through people as suggested by Porter in his studies. Hence, the notion of strategic management at Southwest has been a product of several factors which are best explained as a cycle of events. Firstly, the company succeeds at value creation for its workers which translates to high levels of motivation. Secondly, the company uses the motivation produced by this in order to implement processes that reduce cost and enhance service. Third, the company succeeds at capturing value by offering both low cost and high levels of customer service better than its competitors.... Secondly, the company uses the motivation produced by this in order to implement processes that reduce cost and enhance service (Hallowell, 1996). Third, the company succeeds at capturing value by offering both low cost and high levels of customer service better than its competitors (Hallowell, 1996). The competitors of Southwest have been traditionally associated with hub and spoke networks that enhanced barriers to entry in the 1980’s along with advanced customer relationship management via segmentation and computerized systems (Hallowell, 1996). Research has suggested that mere â€Å"contestability† is not a sufficient condition for strategic management of airlines (Bailey, Graham, & Kaplan, 1986). Contestability is basically the failure of an airline to remain competitive at a particular route simply because there are no barriers to entry and its competitors can easily enter the route to capture the profits (Bailey, Graham, & Kaplan, 1986). The case of Southwest dem onstrates that clearly the company needed a lot more than mere contestability in order to sustain its strategic advantage over the years. This leads to the premise that the high motivational levels of employees made a crucial role in the strategic management initiatives at Southwest. The company’s core target was to offer airline services at the lowest possible costs, even if that means competition with automobiles. The target customer of Southwest is typically a customer who wants warm, co operative staff as well as superior interaction, response all at a low fare. The aim is, therefore, to offer more for less money rather than less for less money. This has clearly differentiated Southwest from its

Project 1 Essay Example | Topics and Well Written Essays - 500 words - 1

Project 1 - Essay Example The density in each case remained the same for the full candy bar and half candy bar. It is due to the fact, that with the change in the volume, the mass of the candy bar also changed and thus the ratio between the mass and volume remained the same. Both the candy bars have lower densities. The densities in full candy bar and half candy bar remained the same in both the two cases. Both the candy bars have higher volumes and lower masses that made the cars less dense. 3. Explain how buoyancy and density are related in why some things float and others sink. To answer this question completely, use additional resources such as the internet or library. MUST cite your source(s). Buoyancy is the property of an object that keeps it floating instead of sinking. Buoyancy and density are closely related to each other as density is the property of an object that enables it float. If the object has fairly large volume with respect of its mass, it will have less density that enables the object to float over water. In the similar manner, if the object has large mass with respect to its volume, it will sink. In order to float over water, the object should have a density lesser than density of water (Editions.sciencetechnologyaction.com). 5. If measured accurately, your densities should be very close to each other when comparing the whole candy bar to the same half candy bar. What factors would influence your calculations between the densities to be completely different (assuming your measurements weren’t close)? Make a

Whistleblowers Case Study Example | Topics and Well Written Essays - 500 words

Whistleblowers - Case Study Example y’s global business environment, white-collar crimes are extremely difficult to prosecute due to the underlying mechanisms available to suppress evidence. In this respect, whistleblowers come in handy. Rather than taking part in the actual misconduct, some individuals in the corporate setting will be enticed to report misconduct subject to the rewards availed. However, caution should be prioritized to avoid faked whistleblowing intended to collect the offered benefits. In general, high rewards will create an incentive for whistleblowers to expose business and corporate misconduct in the economy. Encouraging whistleblowing will undoubtedly have its negative consequences, but the benefits stand a better a chance to outweigh the negatives. To start with, promoting whistleblowing will force business players to adhere and comply with the relevant laws, rules, and regulations (Vandekerckhove 156). In the process, employer-employee relations will move towards enhanced trust and loyalty. In essence, whistleblowing will encourage both employers and employees to follow ethical codes of conduct. Speculating a culture of suspicion and distrust is only probable if misconduct is an underlying practice. Straight and ethical practices should not warrant fear or worry as far as whistleblowing is concerned. In this respect, whistleblowing will encourage trust, loyalty, and straightforwardness in the society. Amid this, the emergence of negatives cannot be ruled out. Whistleblowing could result in tense relations between employers and employees. In any setting where whistleblowing might occur , employees and employers should unite to do what is right, legal, and ethical. Even though the number of pursued whistleblowing cases is significantly low, the need to make penalties stricter is critical. To start with, increasing the penalty will act as deterrence for corporate misconduct (Richter and Frances 147). Persons intending to engage in any form of misconduct will have to come to

Boston Beer Company Essay Example | Topics and Well Written Essays - 2500 words

Boston Beer Company - Essay Example The trend all these ratios have over time is analyzed to check if they are deteriorating or improving (Steffy, Zearley & Strunk, 1974). Comparative valuations of different companies belonging to the same sector are also carried out in order to see how they stack up, and also to get an idea of their comparative performance (Sen, 2008). Ratio analysis is a very important factor of fundamental analysis (Johri, 2010). Although there is abundance of financial ratios, investors are mostly familiar with a few key ratios, especially the ones that are comparatively easy to calculate. A few of these ratios are the current ratio, the debt-equity ratio, return on equity, the price/earnings ratio and the dividend payout ratio. (Muhammad Arif Ghani, 2007) Most companies have some values, for a specific ratio, that have a certain range. A company may be assumed to be grossly overvalued or undervalued depending on the ratio figured out provided the ratio does not fall in that range. Keeping in view the fact that a ratio which is acceptable for one industry may be considered as much more high in another, therefore the ratios are generally compared for those companies which are in operations in the same sector. A good example may be; companies in utilities sectors typically have high debt-equity ratios, but the same ratio may be considered as unsustainably high for a technology company (Palepu, Bernard & Healy, 1996). Ratio analysis can be very useful in providing an early warning of a potential deterioration or improvement in the financial performance or situation of a company. To find for any such hints, the experts and analysts does engage in extensive ratio calculations of the financial data in quarterly financial reports of a company (Cadle, Paul & Yeates, 2010). Generally, successful companies show solid ratios in all the areas, and a small hint of weakness in a single area may

Non-linear Pro vs Quick Takes Video - Nature of Agency Essay

Non-linear Pro vs Quick Takes Video - Nature of Agency - Essay Example Hence, the principal would be vicariously liable for the acts of the agent and would be responsible for all the negotiations and contracts that the agent has signed on his behalf (Herbert, 2010). The agency relationship would be having both commercial and legal effects. For example, an owner of the business would rely on various agents to do the ordinary day-to-day work of the business, whereas since a company is an artificial person, it would be represented through the act of the agents (including management and employers) that make decisions and take responsibilities on behalf of the company. Usually a contract or an agreement may be present between the agent and principal who may be in an oral or written format, and giving agency in an expressive or implied manner. Implied agency refers to acting out of necessity or the situation would dictate that an appropriate person make a decision on behalf of the principal. In a corporation, the senior executives and the management board hav e the implied authority and the apparent authority to make decisions on any matter on behalf of the principal/company (London International University, 2007). ... An agreement for agency may also be mentioned in the employment contract, be a separate agreement of agency, be mentioned in the contract with the third party, etc. Secondly, there is a relationship bringing third parties in contract with the principal, for certain works (Herbert, 2010). For an agent to act on behalf of the principal, he/she should have some authority to do so. If the agent was not acting out of his power and not out of necessity, the agent and not the principal would be held liable. The authority provided to the agent is of 3 types and are recognized by law. These include actual authority, apparent authority and ratified authority. Actual authority involves actual provision of authority to the agent to represent the principal in certain matter and make decisions. The authority provided here may be expressed or implied. If an agent has acted beyond the actual authority, it can be considered as a breach of contract. When an agent is acting within the scope of his empl oyment with the principal, then the claimant can claim from the employer. Ratification is a process in which an agent who had been acting on behalf of others in the past and would have performed certain non-authorized acts, but the same is being approved now (Rao, 2009). Apparent authority or ostensible authority refers to the conduct or words of the principal which would require a person to take decisions on behalf of the principal and act on behalf of the principal with any third party. Here the third party is in a position to believe that the agent is acting on behalf of the principal, even if the issue with acting with this particular third party has never been discussed between the principal and the agency. The third parties have acted within reasonable grounds,

Michelangelos Sistine Chapel Ceiling Essay Example | Topics and Well Written Essays - 500 words

Michelangelos Sistine Chapel Ceiling - Essay Example The project was based on fresco, which was a contemporary complex form of painting. Frescos sometimes required carving and minor sculptures as well. Although the medium was thus a very challenging one, Michelangelo was a versatile genius. He was well acquainted with designing and crafting frescos and he was an expert in this field. However, the main complexities in this project involved altitude and complexity of the subject itself. In order to complement the twelve apostles to be painted across the ceiling, Michelangelo had to create over 300 figures. â€Å"The rectangular panels in the centre depict episodes from the book of Genesis. They are surrounded by prophets and other biblical figures†.What is more, the ceiling was at a height of approximately 68 feet from the floor. The surface of the ceiling was considerably curved. So Michelangelo might have wondered that how exactly his painting was going to be viewed and understood from below. Now on a curved surface, any proport ional two dimensional representations would probably look awkward. For example, if a human figure is made across a curved surface, then some of its portions would emerge nearer to the viewer creating disproportions. Michelangelo apprehended this enormous challenge of visual illusions even before he actually began the work. Consequently, Michelangelo started to create disproportional human figures. For example, if the middle portion of a human figure was to be created across the distal concave part of the ceiling, Michelangelo magnified it.

Phy 155 Assignment Essay Example | Topics and Well Written Essays - 500 words

Phy 155 Assignment - Essay Example Another factor was the market monopoly for large computers held by IBM, which made the product only applicable to private computers. CISCO is a multinational corporation specializing in designing, manufacturing and selling network equipment. The company was started by a married couple, Sandy Lerner and Leonard Bosack , who both worked at Stanford University in computer operations (Schneider, 2010). The company managed to develop high standard routers although it faced lawsuits as some of their software were said to have been stolen from the university. The founding couple worked out of the company in 1990, when the company went public. The company went public and became the most valued companies at more than $500 billion. The company has extensively utilized acquisitions in achieving the tremendous growth it currently enjoys. Despite the acquisitions, the company captured the internet wave through production of modem access shelves and core GSR routers. Large capacity – fibre optics can transmit a large number of conversations simultaneously compared to other cables, fibre optics (up to 1million), coaxial cables (10,000 conversations, microwave radio (2000 conversations. There are approximately over 5 billion internet connection devices in the world today. The current world population is said to be about 7.2 billion people. The number of internet connection devices is expected to surpass the total world population as internet connections continue to increase. The effects of increased internet usage are expected to enhance communication and information transferring between the increased population. The increased usage and increased population is expected to increase the pressure on internet connections necessitating upgrades within short

The Benefits of Home Schooling Essay Example for Free

The Benefits of Home Schooling Essay The education in our public schools has been on the down slope for many years. With the increased amount of drugs, school shootings, and other dementia in these schools, more and more parents are making the choice to home school their children. Although many people claim that public education is better and more suitable for children, many facts and statistics show that home schooling is equally, if not more, beneficial. Home schooling parents have many different reasons for choosing to home school their children. According to the Parent-NHES:1999 (http://nces.ed.gov/pubsearch/pubsinfo.asp?pubid=2001033), almost 50 percent of the home schooled students whose parents were surveyed said that the reason for choosing to home school their children was because their child would have a better education at home. Most children would be able to learn better at home because they are able to learn at their own pace while, in public schools, they have to learn at the pace the teacher sets, which may be too fast or too slow for different students. Also, at home, the child is able to have more hands-on activities and be more in touch with nature and the outside world and even include many of these things in his or her studies. Most parents object when someone teaches their children and idea or theory contrary to theirs or teaches them something the parent does not deem appropriate, which occasionally happens in public school. This is also one of the more popular reasons for parents to choose to home school over public school. Home schooling allow the parent to instill upon their children moral and religious values without having anyone else, such as in public school, try to change that. The children can grow up with the rest of the family in the religious and proper surrounding that the parent provides, with no boundaries as to what they can teach. Most children go to school for 7-8 hours a day with 20-30 students in the classroom and only one teacher. It is very difficult for a teacher to have  one-on-one contact with each student each day. Let us say that an elementary school teacher has 20 children in her class for 8 hours a day, and she wanted to have one-on-one tutoring with each student. That would let her have 24 minutes for each student, which is definitely not enough time for the teacher to get a whole days worth of teaching in. when at home, the child can have one-on-one tutoring all the time and it would take them a whole lot less time than 7 or 8 hours to get through with all their work for the day. Just over a quarter of home schooled students whose parents participated in the Parent-NHES:1999 gave the reason of Poor learning environment at school for choosing to home school their children. A good and safe school environment is a place where teachers are able to teach and students are able to learn in a warm environment, without the fear of intimidation and violence. Students who are afraid to go to school because they feel unsafe or unaccepted by their teachers or peers could find it hard to concentrate on their studying, which would obviously prevent them from doing well in school. Health Canada (Trends in the Health of Canadian Youth. 1999) surveys have recorded that bullying victims are more likely to have low self-esteem, matters are made worse when they are harassed by other students. The public schools can try to lessen this mistreatment, but there is really nothing they can do to stop it completely. Some people who have objections to home schooling argue that home schooled students have low self-esteem because they are schooled at home rather than in public school. In 1992, Larry Shyers conducted a study to test the difference between the self-esteem of 70 home schooled children and 70 traditionally schooled children, both groups between the ages of eight and ten. Using the Piers-Harris Childrens Self-Concept Scale, a regularly used way of measuring self-esteem, there was no difference found between the two groups of children. (Larry Edward Shyers obtained his PH.D. degree at the University of Florida in part by conducting the research reported in his thesis, Comparison of Social Adjustment Between Home and Traditionally Schooled Students.) For many parents, one of the better benefits of home schooling their children is that the family can spend more time together. Most families whose children are in public school never get to spend quality time together, because they never have time. The kids leave for school in between 7:15 and 8:15 and do not return until 3 or 4, or even later if they participate in extracurricular activities. Then they come home and they have to do their homework and there is barely any time left for family time. Almost 17 percent of home schooled students are home schooled for this reason. A great benefit of home schooling is the flexible schedule. Vacations and other outings can be planned for times when the family is ready and oftentimes when the crowds are smaller and the costs are lower. The children do not have to worry about missing school and having to make up work because they can easily take their work along with them or just make it up later. Also, with the flexible schedule, the children can learn at their own pace. If they understand something completely, they can just breeze right through it. IF they do not understand something else very well, they can take as much time as needed for them to understand. In the same year that Larry Shyers completed his doctoral degree thesis research on home schooling socialization, Thomas Smedley completed research for a masters degree at Radford University in Virginia with a similar experiment (Socialization of Home School Children A Communication Approach. http://www.members.aol.com/to Msmedley/smedleys.htm). Smedley states that John Dewey, the Father of modern public education, emphasized the fact that socialization is the primary goal of the educational process. Smedley referred to Deweys Moral Principles in Education, stating that if Deweys conviction was summarized as a research hypothesis, it would read: Public education aims for, and will result in, great social competence for children On page 11 in Deweys book, he said that, Apart from participation in social life, the school has no moral end nor aim. In Smedleys thesis, he investigated the assumption that public school  teaches students socialization better than home schooling. The subjects of this experiment were 33 children, 20 home schooled and 13 public schooled. He evaluated the childrens socialization and daily living skills using the Vineland Adaptive Behavior Scales. The scores the children received when combined, reflected their maturity levels. After all data was processed using the Statistical Program for the Social Sciences, it was shown that home schooled children were much better socialized and more mature than the public schooled children. The overall conclusion we would come to is that home schooling parents are providing the adequate social needs for a child. Socialization is the big opposing factor when people are considering home schooling their children. Many people think that home schooled children do not have many friends, but that is only true if that is what the parents choose for their children. Since home schooled students do not have friends in school with them, they find other places to socialize, such as church, Scout groups, athletic teams, home schooling support groups and other places. They also find other ways to spend their time, such as volunteering in the community, like working at nursing homes or the libraries. By participating in the community, home schooled children are around many different people of different backgrounds and ages, so they are very comfortable with just about anyone. For most home schooled children, doing their work for the day only takes a few hours compared to the 7 or 8 hours in public schools; there are no questions from the parents about whether the learning environment is safe or whether the children are being taught something contrary to what they believe; the schedule is very flexible; they have better speaking skills and are more comfortable with being around a variety of people rather the public schoolers; and there are many more benefits than those discussed in this report. But hopefully, those examples will be enough to show that home schooling really is equally, if not more, beneficial.

Travel and Tourism Essay Example for Free

Travel and Tourism Essay Inbound What is inbound travel? This is when people from other countries come and visit the up and all around the UK for a holiday or the purpose of tourism. For instance a person from Spain coming to visit the UK on holiday Domestic What is domestic travel? This is when UK residents travel around the UK for the sole purpose of tourism. For instance a person from Halifax going on holiday to Blackpool or Whitby The difference within the both inbound and domestic is that people from out of the UK come in to travel and become tourists in the UK and with domestic is when people travel around the UK for a holiday like going to Blackpool etc. A couple from china that would want to visit all of the main capitals in the UK. As the couple from china may have never visited the uk they would not know what to do like getting information or the best places for accommodation therefore I will be explaining everything where to get information from and where to visit. The United Kingdom has four main capital cities: London Edinburgh Belfast Cardiff The Chinese couple would wish to visit all of these main capital cities. When going to another country getting information is key about the city but about everything in general like getting information or finding out where to go the best places to stay etc. and good and an effective website because it has everything that an tourist would need the website is easy to navigate there are maps and brochures that the couple could print of and there is also an 360 degrees virtual map that they could use to get familiar with  London. They also have a customer’s service desk so that if the couple ever needed an information then they would be able to ring them Where to stay within time London has become very successful within the tourist business. See more: The issues concerning identity theft essay What to do? whilst London is an very modern and well developed the city still has its world renown heritage sites that tourists can visit there are over 2123 attractions that they could visit whilst they were in London these consist of the major attractions like the big ben tower of London or even the London eye all of these activities can be booked online and may be cost less than what it would cost to buy the tickets on the day. On the visit London website they do an attraction planner that the couple could download and print so that they can plan there whole activities and that they can get enough activities and attractions that they6 would need to. They are attractions for the couple to see that they may not have to pay for and that they can enjoy for free. The London nightlife in best in the whole country they have the best night clubs in the uk there so if the couple want to go out or if they don’t want to go to the clubs they can go on romantic dinners and there’ s spas all around London 5* Transport getting around London is very easy due to the many different forms of transport there are taxis there are trains cable cars buses and the tube theses are accessible all around the uk and they are very reasonably priced and they are not very expensive at all. For the couple the cheapest thing for them to get would be the oyster card this is an smart card that can be bought on the website and it can be used all around the uk and how many times they would want to it can be used on any time of transport. The couple can buy these cards online and they can also pay for these and they would get theses in advance for when they plan to travel London On the website visit London they have an whole link that shows disabled people how to access London quicker there are special tours that disabled people can access then there are the transport services there is help for them there and the fares are an lot cheaper every  attraction in London has disables toilets and easy accessible stairs or lifts that they would be able to access. For the customer service there is an 24 hour customer help lines that tourist can ring if they are in any types of trouble the number can be found on the visit London website there is also an map so that people don’t get lost their however they are tourist centres all around London so people can go in If they have an questions. There are many places where the couple could go if they wanted to save some money there are the free attractions around London like the museums etc. or if he couple decide to visit the uk on the visit London site there is an navigation below I have an image of the website its shows any types of special offers like discounts on theatre clubs or there is also an list of cheap hotel offers. The cost to vist the uk can vary depending on wwhere abouts in they would want to vistype of holiday for intance an romantic holiday ect below I am going to show the cost pof visting london for 2 nights 3 days Flights- £470 per person - £940 Hotels  £29 per night - £58 Ativities they are many things to do free activies and then they are the heritage sites 18.80 per person etc Food-shops all around of the uk MAXIMUM  £100 1175.5 Edinburgh Edinburgh is the the main capital city of scotland and is known for its amayzing senory and wonderfull countrey side is an tourist attraction that is normall like by maybe mnature people due to it being quite etc. To accsess information about edinbourgh there are so many sites and the sites are all very easy to navigate and there all really easy to acsess there is inofromation about everything on there like accomadation travel things to do etc one of the websites that I found very usefull and very easy to accsess was the vist edinbourgh website.on this website there was an navigation for everything and it was so eay to navigate there was also lots of pictures so we could see what the senery looks like. Becausde edinbourgh is an capital city and is very well known around the world there acomadation range is endless there is somethin there for everybody is ther couple wanted an romantic hotel or if they wanted an cheap budet hotel the hotels arte really easy to accsess and easy to book. Edinbourgh is an city which is mostly known for its countrey side and there are amny different ways to get around there on the visit london website there are naviagtions of getting around edinbourgh and also navigations about how to get around within edinbourgh there are maps and phone numbers for the toursts so they can accsess the information easlya nd they can plan there jurneys.the most used transport that is sued around edinbouirgh is foot most people prefer walking around edinbourgh however there are other forms of transport like cycling,buses and car hires all these are at an cheap price not to expensive There are many activites that are value for money and attractiosn that the couple could go to and not pay for and all of this activities are again found on the website the activites that they could do would be to vist the beaches or walk thorugh the hils or visit the parks and gardens or maybve the meusems or there are also the churches and catedrals however they are other activeities that may be just as fun where the couple may have to pay for like the edinbourgh zoo or thwre are castles,monetery history or they could vist an amazyinbg attraction called the real king marys close.shopping with in edinbourgh is at an whole different range there are street markets or they are the shopping malls loctaed all around edinbourgh. Custemer service ceneters are located all around the city there are spread out all around the city there are some in the bus and train stationa dn in the shopping centers they are there to help people who may be struggleing or they are lost ther is also accsess to languge books so if the couple have any troubles then they can look through the books and they can also get help there. On all fo the activites there is easy accsaess like less stairs or there are ramps or even lifets there is also different types of accsess for intace if  there was an person who needed something like if they were on an wheel chair then tehyw ould be able to ask the hotel and they would get it there is an link for accses on the vist scotland website. Costs London to edinbourgh on train is  £18.50 per person with is  £37.00 Acomadation 2 nighst 3 days  £120 Activities- £150 Food and drink  £100 Overall cost  £407 Belfast Belfast is located in ireland and is an small but an capital city the city is known all around the world for its amayzing fesivals its picturess senery Because belfast is na capital city there are many diffret websites that the couple could use for information such as the visit belfast webite which was created ansd funded by the government the webite is accurate and everything on the site can be trused so if the couple would like to pay for they holiday before they went to belfast they could trust the website completeley also with the visit belfast website the couple would not ned to use any other website because there is an navigation for everything such as accomadation thing sto do and about belfast the wevbbsite I seasy to navigate and is very easy to understand. Accomadation because belfasrt is not an very big cit they will be an good range however the range wont be as big as it would be in london or edinbourgh and the range they have there is something there for everybody there is the bed and breakfirt there are the romantic hotels and there is something to match everybodys budget.all the couple would have to do would be tio visit the website â€Å"visit belfast† as they can find the range of accomdation. Getting around belfats most people would tend to use foot and if the couple would want to walk around belfast then they would be an seris of maps that they could download and print out or if they would not prfer to wlk then they could go around by taxi or bus or if they would prefer to hire out there own car all they would need to would bring there valid driving licince. Because belfast is an capital city there are custemer service  centers located all around th ecity so if people are lost or in trouble or even want vbasic information they can go in to the custermer service centers however there is an alternative ther can ring the custermer service phone line which is 0 118 500 it also shows number for the emargency services. Things to do in belfast belfast is full of fun educatiinal and amazying things to do there are shopping centers maybe the best in the uk there are asttractions such as the titanic in belfast they have got the titanic tourss,titanic pump and the titanic boat trips abnd the titanic resturants this is all bcuase that’s whtas blefast is known for and its eductaional and intresting if th ecouple would want something different they have alwatys got there famous fesitvals that they could watch freom the free side.if the coupel are luking for an budget holiday they can click on the butten that would say budet and they would find all sorts off amazying activites and attracttiobns that they could do like the botanic gardons or the belfast zoo.food within belfast is one of th bes in the countrey there are resurants alll over there are budget resturants but also very well preserver historic howver they would be an bit expensive so the couple do have na range according to there budget. Costs Edinbourgh-belfast onplane is  £20 per person with is  £40.00 Acomadation 2 nighst 3 days  £100 Activities- £100 Food and drink  £150 Overall cost  £380.00 Vsist cardiff Wales Capital city youll find unique attractions, top class entertainment and quality shopping with a difference all within walking distance. Innovative architecture sits alongside historic buildings and Cardiff Bay offers entertainment for everyone. Information on Cardiff is very easy to find because eat is an capital city however not every website can be trusted therefore I have chosen to use one website why I know this website can be trusted because it is an website that Is funded by the government therefore the information is correct if the couple would want to plan their whole trip  they could use this whole website except for the travel which they would have to use an website such as easy jet or Thomas cook etc. The website is called visit Cardiff it is very easy to understand and easy to navigate Accommodation the accommodation range within Cardiff is very varied there are 5* or bed and breakfast there is again everything according to the couples budget the hotels can be found according to their star and the hotels are very easy to use and to book and again the website is very secure, however there is an smoking law where none of the couple if they do smoke cannot smoke within the hotel they will be an smoking shelter for them to smoke in. Travel-there is many ways to travel around Belfast there are coach’s buses. Trains walking and they can hire cars the travel system are all very easy and very easy to get hold of if the couple would choose to walk there are maps that they could download and use. There are tourist information centres all around the city so that if the couple so need anything they can drop in to the centres of course free of charge and ask the information about everything and anything there I also an number that the couple could save and whenever they need anything they can ring the number the customer service helpline is an 24 hour website and can be used by anybody .Another way they could get information is if they email the centre regarding any quires and the centre will get to back to the couple as soon as possible. Things to do in Cardiff there are many different places that they could visit in Cardiff and they would never be bored there are attractions for everybody and anybody. They could visit the Cardiff Bay is home to the new BBC Wales studios and is the filming location for Doctor Who, Casualty, Wizards vs Aliens Pobol y Cwm.or if they wanted to do some shopping they could visit the queens arcade this is an shopping centre famous all around the uk. Food places around the city are many there are expensive and cheap hotels they even hold the foodie events so people can try different foods from all around the world. Special offers or budget activities they can visit casinos or there are the sporting activities or even the wonderful castles that Cardiff has to offers so in the city there is something there for everyone and the couple would not get bored. Costs Belfast to cardiff onplane is  £30 per person with is  £60.00 Acomadation 2 nighst 3 days  £120 Activities- £80 Food and drink  £1400 Overall cost  £400 Overall cost for the couple to vsit the uk would be  £2362.50 this would inculde everything food activities travela nd accomadation. The couple from the use can come to UK to visit the main sporting even in the work the Olympics this is the ultimate sporting even that they would want to be a part of. There are man y different website about the Olympics that the group could use it’s an event for everybody and it is an event loved and visited by people from the whole world Information about London and the Olympics is very easy to find like anything that they would need the website that I have found and it is an very interesting website is the Olympics visitors guide on this website there is everything that the group could use to come to the uk and visit the uk however this is not only website there are much more website all they would need to type in would be London 2012 Olympics. Accommodation because this is an event from all around the world London tourist numbers will be at the highest there for accommodation may be difficult to find if it is left to last minute therefore booking in advance on websites wo uld be an good start the easiest and the most cheapest way that the group could accommodate would be if they rented out an house it would be an lot cheaper and it would be an lot closer to the Olympic park. Transport to get form the USA to the UK they would need to catch a plane witch would land in one of the airports. Getting around London is an little bot difficult because of all of the people it would be cheaper for the group to travel around London with either the tube or if they wanted to bus and to walk there is maps all around the city and also there is an virtual 3d map on the visit London website. Customer service there is customer service cabins all around the city and they will be more because of the Olympics it’s so easy to gather information and they could not have a problem with  the language anyways because they speak English Activates the main activity that they are going to do is visit the Olympics however in their spare time there is many different things within London so that they can do there are the nightclubs or there is historical attractions like the tower of London or they could go shopping to shops like Selfridges etc. there are food places spread all round the uk for the group to visit and they are some of the restaurants that are really cheap. Total cost per person 1 week flight from America to united kingdom  £1001 Group of 4 people would cost each person  £100 Olympic seats  £1500 Food- £150 Other activities  £100 Getting around  £150 Total cost  £3001 A family wanting to visit a theme park There are many different theme parks that the family could visit there is an whole verity like flamingo lad, Alton towers, pleasure land, Thorpe park and much much more. The theme park that I am going to chose is pleasure beach Blackpool. The reason why I have chosen pleasure beach Blackpool is because there is rides there for everybody there are rids for children and adult also there is a beach close by that they could visit Access to information is so easy the actual pleasure beach website is really good there is information about everything rides food places prices etc. Because the family are going for a day visit they would not need accommodation Transport there are many different ways of getting to Blackpool however because they live in the UK they are UK residents they may be a chance that they drive to Blackpool or if they want to they can get the train instead. Accessibly there is all of that around the park like push chairs so that parents could push their children around th ere are many different perks like being told in advance they can sort out extra attraction so there is something there for everybody. Customer service there is centres all around there is also some within the theme park and there is a big information centre at the entrance of the theme park where there is information about everything. Cost there are family tickets that would be available for the family to UK which would be a lot cheaper than the separate tickets  £64 There is many activates they can do with blackpoll as well as the rides there are activities that they can do like they can go to shows which are all so cheap and gift shops etc. or they can go out on to the beach which is free of cost and they would not be paying for anything at all. Total cost Travel  £50 Food  £50 Tickets  £64 Other costs  £100 Total Costs  £264 A couple who want a quite stay at a city Edinburgh Edinburgh is the the main capital city of scotland and is known for its amayzing senory and wonderfull countrey side is an tourist attraction that is normall like by maybe mnature people due to it being quite etc. To accsess information about edinbourgh there are so many sites and the sites are all very easy to navigate and there all really easy to acsess there is inofromation about everything on there like accomadation travel things to do etc one of the websites that I found very usefull and very easy to accsess was the vist edinbourgh website.on this website there was an navigation for everything and it was so eay to navigate there was also lots of pictures so we could see what the senery looks like. Becausde edinbourgh is an capital city and is very well known around the world there acomadation range is endless there is somethin there for everybody is ther couple wanted an romantic hotel or if they wanted an cheap budet hotel the hotels arte really easy to accsess and easy to book. Edinbourgh is an city which is mostly known for its countrey side and there  are amny different ways to get around there on the visit london website there are naviagtions of getting around edinbourgh and also navigations about how to get around within edinbourgh there are maps and phone numbers for the toursts so they can accsess the information easlya nd they can plan there jurneys.the most used transport that is sued around edinbouirgh is foot most people prefer walking around edinbourgh however there are other forms of transport like cycling,buses and car hires all these are at an cheap price not to expensive. There are many activites that are value for money and attractiosn that the couple could go to and not pay for and all of this activities are again found on the website the activites that they could do would be to vist the beaches or walk thorugh the hils or visit the parks and gardens or maybve the meusems or there are also the churches and catedrals however they are other activeities that may be just as fun where the couple may have to pay for like the edinbourgh zoo or thwre are castles,monetery history or they could vist an amazyinbg attraction called the real king marys close.shopping with in edinbourgh is at an whole different range there are street markets or they are the shopping malls loctaed all around edinbourgh. Custemer service ceneters are located all around the city there are spread out all around the city there are some in the bus and train stationa dn in the shopping centers they are there to help people who may be struggleing or they are lost ther is also accsess to languge books so if the couple have any troubles then they can look through the books and they can also get help there. On all fo the activites there is easy accsaess like less stairs or there are ramps or even lifets there is also different types of accsess for intace if there was an person who needed something like if they were on an wheel chair then tehyw ould be able to ask the hotel and they would get it there is an link for accses on the vist scotland website. Costs London to edinbourgh on train is  £18.50 per person with is  £37.00 Acomadation 1 week  £200 Activities- £150 Food and drink  £100 Overall cost  £487